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nested primers中文是什么意思
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- 中文翻譯
- 造句
- 嵌套引物
- nest n. 1.巢；窩；窟,穴。 2.安息處,休息處；住處,家 ...
- primer primer2 n. 1.裝填火藥者；裝雷管 ...
- nested 巢狀的; 內裝的; 嵌套的; 相互套入的
- degenerated primers 簡并性引物
- deletion-primers 缺失引物
- primers and fillers 底漆及木料填孔漆
- primers for bicycles 自行車用底漆
- primers tubular 管狀底火
- wash primers 耐洗底漿廢料
- allele-specific primers 等位基因特異性引物
- group-specific primers 組特異性引物
- sequence specific primers 序列特異性引物
- zinc-rich primers 富鋅底漆
- nested barges 套裝駁
- nested binding 嵌套結合
- nested block 嵌套分程序; 嵌套塊; 嵌套循環
- nested blocks 嵌套塊
- nested boats 套放救生艇
- nested caldera 巢狀破火山口
- nested cell 嵌套單元
- nested class 巢狀類別嵌套類; 有關
- nested classification 抱窩型分類
- nested code 嵌套碼
- nested conditions 嵌套條件
- nested construct 嵌套構造
- In order to identifiy the virus further , a set of double nested primers for canine coronavirus was selected . the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl , ccvf2 - ccvr2 . the first is a pair of outer primer , and can amplify a fragement of 1086bp . the second is a pair of inner primer . and can amplify a fragement of 515bp . using the nested primers , many ccv strains can be identificated including k378 , insave - l , ccv 1 - 71 etc . synthesizing this set of primers , we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr , and all of them respectively gained two target fragements of 1086bp and 515bp , but the control cell did not
合成該套式引物，選擇大熊貓原代病料和病毒各代細胞培養物，經套式( nested ) rt一pcr擴增，可得到一與設計值5巧bp相符的dna片段，經bst一xl ( 590 ， 1110 )酶切鑒定，證明該擴增片斷為特異性片段;回收大熊貓肝組織原代病料和細胞培養物第2 、 3 、 29代的ccvfz一ccvrz擴增片段，純化，送生物公司測序。
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